Cot-1 RNA expression in pachytene spermatocytes. A

<p>Late pachytene nucleus displaying a low Cot-1 RNA signal on XY. <b>B</b> Late pachytene nucleus displaying a higher Cot-1 RNA signal on XY.<b>C</b> Late pachytene nucleus showing a high degree of H3.1/3.2 nucleosome eviction and a low Cot-1 RNA signal on XY. <b>D</b> Late pachytene nucleus showing an intermediate (mid) degree of H3.1/3.2 nucleosome eviction and intermediate (mid) Cot-1 RNA signal on XY. <b>E</b> Late pachytene nucleus showing a low degree of nucleosome eviction and high Cot-1 RNA signal on XY. <b>F</b> Early pachytene nucleus displaying low Cot-1 RNA expression on XY. <b>G</b> Early or mid pachytene nucleus before H3.1/3.2 nucleosome eviction but with an intermediate (mid) Cot-1 RNA signal. <b>H</b> Histogram showing the relation between the degree of H3.1/3.2 nucleosome eviction and the level of Cot-1 RNA signal (bars) (n = 134, P2, C2,3).