Effect of FOXO3 deficiency on contraction of CD8 T cells.

<p>(<b>A</b>) +/+ and FOXO3−/− mice were infected with LCMV and at the indicated days PI, cells from spleen, liver and lymph nodes were stained with anti-CD8 and MHC-I tetramers. Data are representative of 3 to 8 independent experiments with 4–6 mice/group/experiment for each indicated time point. (<b>B</b>) +/+ and FOXO3−/− mice were infected with LCMV and given a BrdU injection at either 8 or 12 days PI and administered BrdU in drinking water between either days 8–11 or 12–15 PI. At the end of each BrdU pulse (day 12 or day 15), splenocytes were stained with anti-CD8, MHC-I tetramers and anti-BrdU. The percentage of BrdU positive cells amongst tetramer binding CD8 T cells for each pulse (8–11, or 12–15 days PI) was determined by flow cytometry. Data are the mean of at least 6 +/+ or FOXO3−/− mice/group.