TIM-3 is highly expressed on both CD4⁺ and CD8⁺ tumor infiltrating T cells in lung cancer.

<p>Tumor infiltrating lymphocytes (TILs) were harvested from lung cancer tissues, adjacent normal tissues, and peripheral blood monouclear cells (PBMCs) from whole blood of patients. Cells were then stained for CD4, CD8, TIM-3 and PD-1. A. Representative dot-plots show TIM-3 expression on CD4⁺ and CD8⁺ T cells in various tissues from lung cancer patients. Lymphocytes were gated for further analysis of CD4 and CD8 T cells. More than 90% CD4⁺ or CD8⁺ cells were CD3⁺. B. Summarized results of the percentage (%) of TIM-3 expression on CD4⁺ and CD8⁺ T cells from lung cancer patients are shown. Horizontal bars depict the mean percentage of TIM-3 expression on CD4⁺ and CD8⁺ T cells. Error bars: s.e.m. C. Dual expression of TIM-3 and PD-1 on gated CD4⁺ and CD8⁺ T cells is shown. The p-values were calculated using the one-way ANOVA.