Mutation analysis of PIK3CA and KRAS genes in NSCLCs.

<p><b>A.</b> Mutation detection in the exons 9 and 20 of PIK3CA from NSCLC. The negative derivative of the fluorescence (−dF/dT) versus temperature graph shows peaks with different Tm. The wild type sample showed a single Tm at 66°C. The heterozygous mutant sample showed an additional peak at 57°C. <b>B.</b> Point mutation in the PI3KCA gene involving a GAG→AAG transition in codon 545 of exon 9 inducing the substitution of a glutammic acid with a lysine (E545K). <b>C.</b> Point mutations in the KRAS gene involving a GGT→GCT, GGT→TGT, GGT→GTT; GGC→TGC transition in codon 12 of exon 2 inducing the substitution of a glycine by an alanine, a cysteine and a valine (G12A G12C G12V) transition in codon 13 of exon 2 inducing the substitution of a glycine by a cysteine (G13C). <b>D.</b> pAKT staining of sample ADC-30.