Expression of genes that were changed by 3.0-fold or above in PBMCs treated with control medium or cigarette smoke extract (CSE) for 8 hours.

<p>PBMCs were treated for 8 hours with RPMI-1640 medium (control 1–3), 3% CSE (1–3) or 10% CSE (1–3). RNA was extracted from each sample and gene expression values measured using the Illumina HumanRef-8v3 BeadChip array. (<b>A</b>) Heat map representation of normalized signal intensity values for genes altered by ≥3.0-fold. Red denotes high expression and turquoise denotes low expression. Order of samples was dictated by hierarchical clustering. (<b>B</b>) Table showing gene name, fold change and p-value for all genes upregulated by ≥3.0-fold. (<b>C</b>) Table showing gene name, fold change and p-value for all genes downregulated by ≥3.0-fold. Statistical significance (p<0.05) was calculated using student's t-test followed by Benjamini-Hochberg FDR correction on GeneSpring GX11.0.2 software. Fold change represents a comparison between mean normalised signal intensity for control (n = 3) versus smoke (n = 3) treated PBMCs. Refer to Data S2 for full data sets and Entrez Gene IDs.