Knockdown of <i>chd7</i> affects eye and heart development.

<p>(<b>A–B</b>) Representative images of eye phenotypes induced by injection of <i>chd7</i>-MO in zebrafish embryos (<b>B</b>) compared with control embryos (<b>A</b>) at 72 hpf. Lateral view with anterior to right. <i>Chd7</i> morphants had a reduced ocular size. <i>Chd7</i>-MO-injected zebrafish exhibited cardiac anomalies such as dysmorphic heart and tube-like heart shape (<b>D</b>) compared with control embryos (<b>C</b>). Insets in (<b>C</b>) and (<b>D</b>) represent an illustration of the heart phenotype in control or <i>chd7</i> morphant embryos, respectively. (<b>E–F</b>) Bar graphs illustrating the prevalence of eye (<b>E</b>) and heart (<b>F</b>) phenotypes in wild-type (white bar), <i>chd7</i>-MO (black bar), control-MO-injected embryos (grey bar) and mRNA rescue (dark grey bar). <b>***</b><0.001, <b>*</b><0.01. <b>***</b><0.001, <b>*</b><0.01.