Chd7 knockdown leads to somite boundary and segmental vasculature defects.

<p><i>Efnb2a</i> expression in control morphant (<b>A</b>) and <i>chd7</i> morphant (<b>B</b>) zebrafish embryos at the 13-somite stage of development. Top is anterior. Insets in (<b>A</b>) and (<b>B</b>) represent coronal sections through control or <i>chd7</i> morphant embryos, respectively, at the 13 somite stage. Posterior views with dorsal towards the top. Expression of <i>ttna</i>, a segment boundary marker reiterates the defects in segment boundary formation in <i>chd7</i> morphants (<b>E</b>) compared to controls (<b>D</b>). Dorsal views with anterior to the top. Segmental vasculature patterning is defective (arrowheads) in <i>chd7</i> morphants (<b>H</b>) compared to control morphants (<b>G</b>) as visualized using <i>fli1</i>: GFP transgenic zebrafish at 48 hpf. Sagittal views with anterior to the right. Graphs show percentages of animals exhibiting <i>efnb2a</i> expression defects (<b>C</b>), <i>ttna</i> defects (<b>F</b>), or segmental vasculature patterning defects (<b>I</b>). The dark gray bar represents <i>chd7</i> morphants and the light gray bar represents control morphants. Scale bar: 200 µm. <b>***</b><0.001, <b>**</b><0.01.