RsmA binds the <i>algUmucABD</i> polycistronic mRNA.

<p>(<b>A</b>) RT-PCR of adjacent genes in the polycistronic mRNA <i>algU</i>-<i>mucA</i>-<i>mucB</i>-<i>mucD</i>. PCR of cDNA using the primer pairs qalgUF-qmucAR (lane 1), qmucAF-qmucBR (lane 2) and qmucBF2-qmucDR (lane 3) or PCR of RNA using the same primer pairs qalgUF-mucAR (lane 4), qmucAF-qmucBR (lane 5) and qmucBF2-qmucDR (lane 6), M marker. (<b>B</b>) RNA-IP assay of F113 wild-type strain harbouring the pVLT31-rsmAHA plasmid. qRT-PCR of HA-immunoprecipitated RNA (black bar) or IgG-immunoprecipitated RNA (mock, grey bar) using the primer pairs qalgUF-R (<i>algU</i>), qmucAF-R (<i>mucA</i>), qmucBF-R (<i>mucB</i>), qmucDF-R (<i>mucD</i>), qhcnAF-R (<i>hcnA</i>) and qfliCF-R (<i>fliC</i>). The <i>fliC</i> gene was used for normalization.