SadB and GacAS regulate <i>algU</i> expression.

<p>(<b>A</b>) Analysis of the swimming motility of F113 wild-type, mutants in the Gac-SadB-AlgU cascade, and <i>amrZ</i> complementation (p<i>amrZ</i>). Different letters indicate significant statistical differences (p<0.05). At 18 h, F113 wild-type strain swimming halo diameter is 11±0.55 (mm). (<b>B</b>) qRT-PCR expression analysis of <i>algU</i> (black) and <i>amrZ</i> (grey) genes (primers qalgUF-R and qamrZF-R, respectively) in F113 wild-type, <i>gacS</i><b><i>⁻</i></b>, <i>sadB</i><b><i>⁻</i></b>, and double mutant <i>gacS-sadB</i>. <i>16S</i> gene expression (primers 16SF-R) was used for normalization. Different letters indicate significant statistical differences (p<0.05).