<i>DIP13</i> Gene Deletion in <i>T. brucei</i> PCF.

<p>(A) Southern blot analysis of parental line EATRO 1125 (WT) and <i>DIP13</i> double replacement (DKO) parasite lines. Five micrograms of genomic DNA from each parasite line was digested with <i>Sac</i>I, size separated through 0.8% agarose, blotted and hybridised with DIG-labelled DNA probes (∼200 bp) as indicated. Corresponding DNA marker positions are shown (kbp). (B) Growth of PCF cell lines as above monitored over a 96 hour time course. Mean values are shown (n = 3) +/− SD (not all error bars are visible). (C, D) Cell body length (C) and paraflagellar rod length (D) in PCF parental line EATRO 1125 (WT) and <i>DIP13</i> double replacement (DKO) parasite lines. Mean values are shown as horizontal lines (+/− SD) (n = 100). Statistical analyses were performed using an unpaired t test. Asterisks represent statistical significance compared to the parental line (WT) (* = p<0.05). (E) Scanning electron micrographs of parental (WT) and <i>DIP13</i> double replacement cell line (DKO). Bar, 2 µm.