Gene expression analysis of the genes <i>SCO0877</i> and <i>SCO7173</i> by RT-PCR.

<p>Analysis was carried out on <i>S.coelicolor</i> M145 strain as indicated in the Methods section after 28 (<i>SCO0877</i>) or 32 (<i>SCO7173</i>) PCR amplification cycles. RNA was extracted from cultures after growth for 48 h and 60 h in defined (M) and complex (C) MG medium. The identity of each amplified product was corroborated by direct sequencing. The absence of contaminating DNA in the RNA samples was assessed by PCR. Transcription of the <i>hrdB</i> gene (<i>SCO5820</i>) was assessed as an internal control (bottom).