Pre- and post-lyophilization antigen binding and specificity of clone <i>350-E2</i>.

<p>Fresh, non-lyophilized <i>350-E2</i> yeast and lyophilized, rehydrated <i>350-E2</i> yeast were split into 10 aliquots. In Panel A, each aliquot received either no antigen, or 100 nM each of 350, 142, Jacob, or 780. Biotinylated antigens were used in every case, and antigen binding was detected by incubating with SA-PE. Panel B was performed as in Panel A, except that non-biotinylated antigens were used and antigen binding was detected by incubation with rabbit-anti-350 antiserum followed by GaR-PE. The percent of PE-positive yeast was determined by gating the PE fluorescence relative to the “no antigen” control. Values are means of three experiments plus or minus the standard deviation. The light grey bars indicate binding of the fresh yeast to each antigen, and the dark grey bars indicate binding of the lyophilized yeast to the antigens.