DPEP1 overexpression enhances sensitivity to gemcitabine.

<p>DPEP1 overexpressing cells and control cells were analyzed for cellular sensitivity to gemcitabine using Panc1 (A) and MIApaca2 (B). Overexpession of DPEP1 increased the sensitivity of Panc1 and MIApaca2 cells to gemcitabine. Control cells are Panc1 or MIApaca2 cells transfected with GFP control vectors. Cells were treated with Gemcitabine for 96 hours at different doses. The MTS assay was used to quantitate cytotoxicity (cell death) according to the manufacturer's instructions. Relative cytotoxicity (%) was calculated using the formula: [1−(OD₅₇₀ of drug treated cells/OD₅₇₀ of untreated cells)]×100%. Data are means ± S.D. from 3 independent experiments. * T-test <i>P</i><0.01.