ENU-induced mutation that protects mice against <i>P. berghei</i> ANKA-induced cerebral malaria.

<p>(<b>A</b>) Breeding scheme for the production and identification of ENU-induced recessive mutations that convey protection against cerebral malaria (CM). Details of the breeding strategy are described in “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031012#s4" target="_blank">Materials and Methods</a>”. G3 and F2 pedigrees were phenotyped for the presence of animals resistant to <i>P. berghei</i>-induced CM. (<b>B</b>) Mice were infected with <i>P. berghei</i> ANKA (10⁶<i>P. berghei</i> ANKA-parasitized red blood cells, i.v.) and survival was monitored over time for individual pedigree P48 G3s (green and blue lines) derived from independent G2 females and a G1 male, and for susceptible C57BL/6J (B6, red line), and resistant mutant mouse strains bearing loss of function mutations in either the IFN-γ gene KO (IFN-γ KO, black line) or IRF8 (BXH2, brown line). Mice surviving past day 13 post infection were considered to be CM-resistant.