Expression of <i>Brg1</i>, <i>Brm</i>, and <i>Wap</i> in CD45⁻Ter119⁻CD31⁻ mammary gland subpopulations.

<p>A. Representative FACS plot of the subpopulations remaining after removing the hematopoietic and endothelial (CD45⁺Ter119⁺CD31⁺) cells from a suspension prepared from dissociated mammary tissue from a normal adult virgin mouse. The CD24⁺CD49f^low/− subset is enriched in luminal (Lum) cells; the CD24⁺CD49f⁺ subset is enriched in mature myoepithelial (Myo) cells; and the CD24⁺CD49^high subset is enriched in mammary stem cells (referred to as mammary repopulating units or MRU). B, C. RT-qPCR analysis of <i>Brg1</i> (B) and <i>Brm</i> (C) mRNA levels normalized to <i>Gapdh</i> levels in wild-type, flow-sorted mammary cell populations from adult virgin female mice. The Lum, Myo, and MRU subsets are as defined in panel A. Each histogram represents the mean ± SE from 3 independent experiments. D. RT-qPCR analysis of <i>Wap</i> mRNA levels normalized to <i>Gapdh</i> mRNA levels measured in the same 3 subsets. Lum, Myo, and MRU cells were isolated from pregnant females (E17.5 and E13.5) and virgin/nulliparous females (for representative FACS plots, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031346#pone-0031346-g001" target="_blank">Figure 1</a>). Asterisks indicate that the <i>Wap</i> signal was below the limit of detection. Each histogram represents the mean ± SE from 2 (E17.5) or 3 (E13.5, virgin/nulliparous) independent experiments.