In vivo-generated Th2 cells store and mobilize pCD40L.

<p><i>A</i>, BALB/c mice that had received 4get/DO11.10 CD4⁺ T cells were subcutaneously immunized with papain plus OVA protein. Seven days later, cells from dLNs were analyzed by the mobilization assay. <i>B</i>, Gating strategy for antigen-specific IL-4/eGFP⁺ DO11.10 cells (CD4⁺IL-4/eGFP⁺KJ1-26⁺). <i>C</i>, Mobilization of pCD40L by IL-4/eGFP⁺ DO11.10 cells in response to PMA plus ionomycin or OVA peptide-pulsed APC. <i>D</i>, Those cells were further differentiated as T_FH (CXCR5^hiPD-1^hi) and Th2 (CXCR5^lowPD-1^low) cells. <i>E</i>, Mobilization of pCD40L by T_FH and Th2 cells in response to PMA plus ionomycin. <i>F</i>, To obtain polyclonal Th2 cells, mice were sensitized with OVA/alum followed by intratracheal challenge with OVA/PBS. <i>G</i>, The gating strategy for Th2 cells (CD4⁺T1/ST2⁺) and non-Th2 cells in BALF. <i>H</i>, Mobilization of pCD40L by polyclonal Th2 and non-Th2 cells in response to PMA plus ionomycin. Data are representative of two to three independent experiments.