Knockdown of Sox10 leads to inhibition of migration of B16F10 melanoma cells.

<p>B16F10 murine melanoma cells were transfected with control siRNAs, MT1-Sox10 (A, B; MT, mutant) and MT2-Sox10 (E, F) or siRNAs specific for Sox10, WT1-Sox10 (C, D; WT, wild type) and WT2-Sox10 (G, H). Nuclei were stained with DAPI (A, C, E, G) and anti-Sox10 antibody (B, D, F, H). Nucleotide sequences of MT1-Sox10 and MT2-Sox10 differ from those of WT1-Sox10 and WT2-Sox10 by 5 nucleotides respectively (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031477#pone.0031477.s007" target="_blank">Table S1</a>). Sox10 was down-regulated only with WT siRNAs (D, H) but not with MT siRNAs (B, F). B16F10 cells treated with siRNAs were put to transwell migration assay (I–M). Transfection of WT1-Sox10 (K) and WT2-siRNA (L) led to significant reduction in migration of the cells compared to transfection of MT1-Sox10 (I) or MT2-Sox10 (J). (M) Quantitation of transwell migration assay. The effect of Sox10 knockdown on the number of cells that migrated through the filter pores is shown in percentile relative to the matching control case. Values represent the average of 5 independent trials, and error bars represent standard deviations. The asterisk (*) represents a significant difference with the <i>p</i> value of <0.05.