Confirmation of microarray expression profiling.

<p>(A) Quantitative real time RTPCR assays were carried out using B16F10 cells transfected with the MT1-Sox10 or WT1-Sox10. A subset of genes that showed down-regulation by WT1-Sox10 in the microarray assay by 2.5 fold or higher in all triplicates (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031477#pone-0031477-t001" target="_blank">Table 1</a>) and two non-target genes whose expression levels were unchanged (Ald1a and Ctbp1) were used to validate the results from the microarray assay. The effect of Sox10 knockdown by the specific siRNA on the expression level of each target gene is expressed relative to that of the control siRNA after normalization with GAPDH expression level. Values represent the average of three independent real-time PCR experiments each carried out in duplicates, and error bars represent standard deviations. (B) Real time RTPCR carried out with MT2-Sox10 and WT2-Sox10. The asterisk (*) represents a significant difference with the <i>p</i> value of <0.05.