Lack of cholesterol leads to loss of APL-1::GFP in head neurons.

<p>A) Representative pictures of APL-1::GFP expression in three head neurons of L4 worms grown for one generation on plates with (+) or without (−) cholesterol. Head neurons are identified by A (anterior), P (posterior) or C (central). B) Quantification of APL-1::GFP fluorescence (n = 15 per experiment). C) Representative pictures of APL-1::GFP expression in L4 worms of the second generation grown on normal or cholesterol-deficient plates. D) Quantification of the neuronal cell body expression of APL-1 (n = 10–15 per experiment). Experiments were performed twice. (*, P<0.05) Error bars represent the s.e.m.