Mouse-adapted NS1 mutations affect IFN-β production and viral yield in the mouse lung.

<p>Groups of 6–12 female CD-1 mice were infected intranasally with 1×10⁵ PFU dose of rHK NS mutants or rHK-wt or mock-infected with PBS. Lungs were collected 1 and 3 days pi, homogenized, and quantified for IFN β concentration using a commercial murine IFN β ELISA kit (left panel), and for viral titre by plaque assay on MDCK cells (right panel) Data represent the means ± SD (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001; two-tailed unequal variance student's t-test).