Parvalbumin and GAD67 co-localization.

<p>(<b>a</b>) The color of the anti-parvalbumin image was inverted and further superimposed onto the anti-GAD67 image (bottom). Parvalbumin expression only cells would appear as bright spots. However, there were no parvalbumin-positive cells that did not express GAD67. Scale bar: 500 µm. Deep layers (A) and the middle layers (B) were visualized at a higher magnification (<b>b</b>). GAD67 expression only neurons became darker spots in deep layers (A) of frontal cortex (black arrow heads). Co-localization between GAD67 and parvalbumin in the middle layers (B) of motor cortex (red arrow heads). Scale bar: 100 µm.