Flow cytometric analyses of NKT cells in adipose tissue in mice fed an SFD or an HFD.

<p>(A) A representative flow cytometric dot-plot defining the population of α-GalCer/CD1d dimer⁺TCRβ⁺ and NK1.1⁺TCRβ⁺ cells in adipose tissue of SFD-fed mice (a, c) and HFD-fed mice (b, d). (B) The proportion of each subset in SVF. NK1.1⁺TCRβ⁺ cells (a), CD4⁺ (b), CD8⁺ (c), and CD4⁻CD8⁻ (d) NKT cells (n = 3–6 female mice in each group). Representative data of three similar experiments are shown. The results are expressed as mean ± s.d. Statistical analysis was performed according to the Tukey-Kramer test. *<i>p</i><0.05, **<i>p</i><0.01. (C) The correlation between BW and the percentage of NKT cells in SVF by Pearson's correlation coefficient (R²) test. WT (a), <i>P</i> = 0.000006; Jα18^−/− (b), <i>P</i> = 0.004; and CD1d^−/− (c), <i>P</i> = 0.2. Values with <i>P<0 .05</i> were considered statistically significant.