Immunogenicity of MVAΔC12L in C57BL/6 mice.

<p>Groups of four C57BL/6 mice were i.p vaccinated with 5×10⁷ pfu of MVAwt (white bars) or MVAΔC12L (black bars) and seven dpi T-cell responses against VACV peptides B8R (CD8⁺-specific), E9L, H3L and L4R (CD4⁺-specific) were evaluated in the spleen. (<b>A</b>) The magnitude of the response was measured by IFN-γ Elispot assay after 24 hr stimulation. Background (RPMI negative control) subtracted results are depicted as mean IFN-γ spot forming units (SFU) per 10⁶ splenocytes ± SD. (<b>B</b>) IFN-γ production in splenocyte-culture supernatants was evaluated by ELISA after 72 hr stimulation. (<b>C</b>) Quality of the response was analyzed by ICS. Degranulation of specific-CD8⁺ T-cells was assessed with CD107a/b mAb together with IFN-γ production, after 5 hr stimulation with the indicated peptides. Results are expressed as mean % of specific CD8⁺ T-cells ± SD. Statistically significant differences: *p<0.05, **p<0.01, ***p<0.001.