Reduced Syk expression in macrophages from Syk^flox/flox/LysM-Cre mice.

<p>(<b>A</b>) Total RNA was isolated from BMDM of Syk^flox/flox/LysM-Cre(−) (WT) and Syk^flox/flox/LysM-Cre(+) (Syk^−/−) mice, and RT-PCR with Syk and GAPDH primers was performed. (<b>B</b>) Lysates of resident peritoneal macrophages (PM) and BMDM were run on SDS-PAGE, transferred to a membrane and immunoblotted with anti-Syk and anti-GAPDH antibodies. (<b>C</b>) WT and Syk^−/− BMDM were incubated with 50 µg/ml mmLDL (to induce macropinocytosis) and 200 µg/ml of native LDL (lipid carrier) for 40 hours. The cells were stained for neutral lipid with Oil Red O.