Replacement of DCs by monocytes and neutrophils upon DC depletion.

<p>(<b>A–D</b>) Mice were treated daily with diphtheria toxin (DT) (<b>A and B</b>) starting one day before infection and were injected with 5×10⁴ Ye pYV⁺ for 24 h (<b>C and D</b>). (<b>A</b>) Representative dot plots showing analysis of monocytes (left) and neutrophils (right) in spleen from control (black) and DC-depleted (red) mice. Numbers adjacent to outlined areas indicate frequency of monocytes and neutrophils. Graphs show the frequency of monocytes (left) and neutrophils (right) per spleen. (<b>B</b>) Bioplex assay or ELISA of chemokine concentrations in sera from control and DC-depleted mice 24 h after DT-treatment. (<b>C</b>) Flow cytometry analysis of the frequency of monocytes (left) and neutrophils (right) in the spleen at the indicated times post Ye infection. (<b>D</b>) Bioplex assay or ELISA of chemokine and cytokine concentrations in sera from control and DC-depleted mice 24 h after Ye infection. Each symbol represents an individual mouse; small horizontal lines indicate the mean ± SD. * indicates statistically significant differences between control and DC-depleted mice (Student's <i>t</i>-test). Data are from 5 (<b>A</b>) or one representative out of 2 or more (<b>B</b>, <b>C</b> and <b>D</b>) independent experiments.