Inactive Clb2 is not required, whereas Polo inactivation is sufficient for Cdc14 re-sequestration to the nucleolus.

<p>(<b>A</b>) Cells of the triple-deletion strain <i>cdc20Δ pds1Δ cdh1Δ</i> arrest in telophase with Cdc14 released from the nucleolus. Simulation was done by setting to zero the rate of synthesis of Pds1, the total concentration of Cdh1, and the initial conditions of Cdh1, Pds1 and PE complex (<i>k</i>_s,pds = <i>CDH1T</i> = 0). (<b>B</b>) After 6 hours of telophase arrest, <i>cdc20Δ pds1Δ cdh1Δ</i> cells are subjected to Sic1 overexpression, and Cdc14 does not completely return to the nucleolus. Simulation was done as in panel A; after 6 hours <i>INH</i> was set to 5 to implement Cdk inhibition by Sic1. (<b>C</b>) In <i>cdc20Δ pds1Δ cdh1Δ</i> cells arrested in telophase, deprivation of Cdc5 causes return of Cdc14 to the nucleolus. Simulation was done as in panel A; after 40 min the rate of synthesis of Cdc5 was set to zero and the basal degradation rate of Cdc5 was increased 10-fold (<i>k</i>_s,polo = 0, <i>k</i>_d,polo = 0.1).