Validation of the role of A and B regions using Heterogeneous Inbred Families (HIF).

<p><b>A.</b> Synthesis of the epistatic interactions involving either the bottom of chromosome 1 (A region) and/or the top of the chromosome 3 (B region) for root related variables. The markers involved in the interactions are colored in blue or red depending on the allele that increases the value of the trait, Sha or bay respectively. <b>B.</b> Genetic map of the RILs that were selfed to produce HIFs from residuals of heterozygosity in F6 generation of the RILs. Blue and red is for Sha and Bay alleles respectively. Yellow show heterozygous regions in RILs in F6. These regions are then fixed in the HIF progeny. <b>C.</b> Validation of the presence of two root QTLs on chromosome 1 and 3 using the four HIFs generated. The percentage of change of the variable induced by Bay allele compared to the Sha allele at the segregating region is indicated with the <i>t-test</i> p-value associated. Data shown gather harvests performed from 20 to 24 days after sowing. The number of individuals and experiments analyzed is indicated.