Comparison of the effect of Xiaflex® and Collagenase A on cell cycle regulation.

<p>Cell cycle gene (PCNA, Cyclin D1 and Cyclin D2) were assessed at mRNA and protein levels using qRT-PCR and In-Cell Western blotting respectively. <b>A.</b> mRNA steady-state levels of cell cycle genes (PCNA, Cyclin D1 and Cyclin D2) after treatment with Xiaflex® and Collagenase A at various concentrations as indicated in the graphs. All the cell cycle genes were dose-dependently down regulated by Xiaflex® and Collagenase A compared to the untreated control group. <b>B.</b> Relative protein expression of cell cycle proteins (PCNA and Cyclin D) after treatment with Xiaflex® and Collagenase A. *p<0.05, **p≤0.01, indicate significant difference in treated group compared to untreated control group. The data presented here are the average of three independent experiments performed in triplicate. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031430#s3" target="_blank">Results</a> are presented as means ± SEM of triplicates.