Relative transcript levels in flower buds assessed at different regions of the <i>bobt_KR</i>-<i>cob</i> co-transcript.

<p>(Relative) transcript level at the <i>atp1-cob</i> junction and <i>cob</i> is more than twice that in the unknown region of <i>bobt_KR</i>. No signal was detected when RNA instead of cDNA was added to qPCR reaction mixture, which excluded contamination of RNA samples with genomic DNA. (A) Expression is presented as the ratio of the accumulation of PCR product for the specific region and the mt 18S rRNA reference gene and (B) copy numbers of different regions of <i>bobt_KR</i>-<i>cob</i> DNA as a ratio of the accumulation of PCR product for the specific region and the mt 18S rRNA reference gene. Whereas target gene and reference copy numbers are approximately equal, <i>atp1-cob</i> cotranscript and <i>cob</i> transcript levels are more than one order of magnitude lower than mt 18S rRNA. The black lines below the gene indicate the positions of PCR products used for quantitation. The standard deviation was calculated on the basis of 5 sibling pairs F and H. The significant differences (t-test P<0.05) between F and H are marked by **.