TAK1 controls wound closure and invasion on type I Collagen gel in MCs from peritoneal effluent.

<p><b>A</b>, Effect of TAK1 inhibition on wound closure. Left, MCs from a patient undergoing PD were allowed to reach 100% confluency in a 6 well plate. MCs were pre-treated with DMSO or NP-009245 (600 nM) for 24 h in culture medium supplemented with 10% FCS. A scratch wound was created on the cell surface using a micropipette tip. The wound area was photographed every 30 min for 24 h by bright field microscopy (4× magnification). The width of the wound was measured and the wound closure rate was calculated. 9 different points from 3 different scratches were analyzed per condition. Only the beginning and the end of the test analysis is shown. The result described is representative of 5 independent experiments. Right, quantification of the experiment described before. P *<0.05 compared to DMSO-treated cells. <b>B</b>, Effect of TAK1 and MEK1 inhibition on MCs invasion on type I Collagen gel. MCs were pre-treated with DMSO, NP-009245 (600 nM) or CI-1040 (2 µM) for 24 h. Cells were allowed to invade type I Collagen gels for 24 hours. Invading cells were fixed and nuclei were counted. Nuclei were counted in ten fields per sample using a fluorescence microscope (40× magnification). Each experiment was carried out in duplicate, and at least 5 experiments were performed. P *<0.05 compared to DMSO-treated cells.