Effect of cytochalasin D on the uptake of AF-SWCNTs by PMs and PLE cells.

<p>PMs and PLE cells were cultured in 48 well culture plates in the presence or absence of 2.5 µg/ml cytochalasin D for 1 h. Fluorescence probe tagged AF-SWCNTs 5 µg/ml) were added and incubation continued for an additional 24 h. At the end of the incubation period, cells were washed with PBS, harvested by trypsinization and analyzed on FACS. Representative flow histograms for AF-SWCNTs uptake in control and cytolchalasin D treated PMs and PLE cells are shown in panel A where cells in M2 window represent AF-SWCNT⁺cells. Bar histograms in the lower panel represents Mean ± SEM of results obtained from 3 independent experiments.