ITPA protects against HAP-induced apoptosis.

<p>ITPA knockdown sensitizes cells to HAP-induced apoptosis. As compared to the control and non-targeting shRNA transfected cells, ITPA knockdown cells undergo approximately 30–50% apoptosis upon HAP treatment for 24 hours. Hydrogen peroxide treatment (0.1 mM, four hours) was used as a positive control. The difference between control cells and cells with the <i>ITPA</i> knockdown is highly significant (***p<0.001, ****p<0.0001). There was no difference between the control versus the non-targeting cell lines in all HAP doses tested. No significant difference in hydrogen peroxide-induced apoptosis was observed for all three cell lines.