Decreased leptin sensitivity and increased lipogenesis in HFD-fed adip-crePTP1B^−/− mice.

<p><i>A</i>: Leptin sensitivity, as measured by the percentage change in food intake after leptin administration in chow-fed adip-crePTP1B^−/− (<i>n</i> = 5) and fl/fl control mice (<i>n</i> = 5). <i>B</i>: Leptin sensitivity in HFD-fed adip-crePTP1B^−/− (<i>n</i> = 3) and fl/fl control mice (<i>n</i> = 3). <i>C</i>: Basal lipogenesis in chow and HFD-fed adip-crePTP1B^−/− (<i>n</i> = 3) and fl/fl control mice (<i>n</i> = 3). <i>D</i>: Insulin-stimulated lipogenesis of chow-fed adip-crePTP1B^−/− (<i>n</i> = 4) and fl/fl control mice (<i>n</i> = 4). <i>E</i>: Insulin-stimulated lipogenesis of HFD-fed adip-crePTP1B^−/− (<i>n</i> = 3) and fl/fl control mice (<i>n</i> = 3). White squares = chow fl/fl leptin; white triangles = HFD fl/fl leptin; black squares = chow adip-crePTP1B^−/− leptin; black triangles = HFD adip-crePTP1B^−/− leptin. White bars = fl/fl; black bars = adip-crePTP1B^−/−. Data are represented as mean ± SEM; Data were analyzed using a two-way ANOVA with Bonferroni multiple comparisons post-tests (*<i>P</i>≤0.05; ***<i>P</i><0.001).