PCR-Temporal temperature gradient gel electrophoresis (TTGE) fingerprinting of the intestinal microbiota of rainbow trout (<i>Oncorhynchus mykiss</i>).

<p>Comparison of the TTGE profiles based on the amplification of the V3–V4 region of the 16 S rRNA genes from DNA extraction (D) and RNA extraction (R) from different individuals (I) from Family F1, which were fed either the control diet D1 (where 100% of the protein in the diet was provided by fish meal and 100% of the oil was provided by fish oil), diet D2 (where 50% of the protein in the diet was provided by fish meal and 50% was provided by vegetable meal (corn, sunflower and soybean meal), or diet D3 (where 50% of the oil was provided by fish oil and 50% was provided by rapeseed oil). Letters indicate some of the bacterial phylotypes described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031335#pone-0031335-t002" target="_blank">Table 2</a>. W: wheat component; S: <i>Sphingomonas</i>, R: <i>Ralstonia</i>, Ce: <i>Cellulomonas</i>, Pd: <i>Pedobacter</i>, B: <i>Blastococcus</i>, L: <i>Lactobacillus aviarus</i>, Lb <i>Lactobacillus</i> sp., St: <i>Streptococcus</i>, Ch: <i>Chelatococcus</i>, Sr: <i>Sinorhizobium</i>, P: <i>Paracoccus</i>, N: <i>Novosphingobium</i>.