Schematic representation and alignment of wild type and transgenic <i>P.berghei</i> circumsporozoite proteins.

<p>(A) Schematic representation of the CS proteins present in each of the four transgenic <i>P. berghei</i> parasite lines generated. The corresponding names of the transgenic parasite lines are shown on the right. PbCS^DHFR parasites carry the wildtype PbCS coding sequence (white boxes, RI and RII indicated with light and dark green respectively) and, similar to all transgenic lines, contain the <i>T. gondii</i> DHFR drug selectable marker inserted in the CS locus. PgCS^SX parasites carry the full PgCS coding sequence (grey boxes, RI and RII indicated with light and dark blue respectively), but with the <i>Spe</i>I (S) and <i>Xho</i>I (X) restriction endonuclease sites inserted on either side of the repeat region. PgCS/Pb^RR parasites contain the PgCS N-terminal and C-terminal regions (grey boxes) and the PbCS repeat region (white box). PbCS/Pg^CT parasites carry the PbCS N-terminal and repeat regions (white boxes) and the PgCS C-terminal region (grey box). (B) Alignment of the wild type PbCS and transgenic PgCS^SX and PgCS/Pb^RR amino acid sequences. Shaded boxes represent areas of amino acid identity. Regions I and II (black lines), the repeat region (blue line) and the <i>Spe</i>I and <i>Xho</i>I restriction sites (red lines) are labelled. The <i>Spe</i>I and <i>Xho</i>I sites were introduced into the PgCS sequence to mediate exchange of the PgCS repeat region with the PbCS repeat region.