Rest regulates recruitment of PRC1 and PRC2 at specific genomic loci.

<p>(A) Western blot analysis of <i>Wt</i> and <i>Rest</i>−/− mES cell lysates. Histones were extracted using LSB. (B) ChIP-seq profiles for Rnf2, Suz12 and H3K27Me3 levels in <i>Wt</i> and <i>Rest−/−</i> mES cells for three Rest target genes (<i>Brunol6</i>, <i>Best2</i> and <i>Prrxl1</i>) and as control, one Polycomb target gene with no Rest binding site (<i>Gjb2</i>). Direct ChIP for the three Rest target genes shown in (B) comparing <i>Wt</i> and <i>Rest−/−</i> mES cells (primer positions are indicated by arrow heads in B and K27 = H3K27Me3) and mRNA levels (C). Error bars represent standard deviations calculated from triplicate qPCR reactions.