The Sp1 <i>cis</i>-acting element is critical for LANA-mediated enhancement of Aurora A transcription.

<p>(<b>A</b>) HEK293 cells were co-transfected full length Aurora A promoter or its mutants driving reporter plasmid with either pA3M-LANA or pA3M vector. At 24-hrs post-transfection, cells were harvested and subjected to reporter assay. The schematic representation of full length Aurora A gene promoter or its mutants-driven luciferase constructs is shown at the bottom panel. The results were presented by the RLU (relative luciferase unit) fold compared to pGL3-basic with vector alone. Data is presented as means±SD of three independent experiments. (<b>B</b>) ChIP analysis of endogenous Aurora A promoter in BC3 cells with or without LANA knockdown. ChIP analysis was conducted using normal mouse IgG, α-E2F, α-Sp1 agarose, and subjected to PCR analysis using primer F(−400) and R(−60) indicated in A. The results of quantitative real-time PCR are shown at the bottom panel.