Increasing cell size results in proportional increase of initiations for both <i>E. coli</i> and <i>B. subtilis</i>.

<p>(A & B) Scatter plots of cell size versus replication origins. (A) An <i>E. coli</i> or (B) <i>B. subtilis</i> strain either overexpressing or depleting the essential division protein FtsZ (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002549#pgen.1002549.s003" target="_blank">Figure S3</a> for FtsZ expression levels). Cell origin numbers were determined by examining DNA content post replication run-out by flow cytometry. The median of the population (counts >200) was used for size. Error equals one standard deviation (n = 3). Red circle in “A” denotes <i>E. coli</i> cells that are smaller than wild type due to increased levels of FtsZ.