Fungal-induced cell growth inhibition reflects cell cycle impairment and aneuploidy.

<p>(<b>A</b>) Representative flow cytometry histograms of cell cycle distribution of mock-treated or <i>C. neoformans</i>-infected (KN99α, 2 independent infections) J774 cells after 48 h, assessed by propidium iodide incorporation (n = 6). X-axis shows intensity of fluorescence and Y-axis number of cells. 30000 total events were acquired per sample with identical parameters of acquisition for all samples and data were analysed as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002555#ppat.1002555.s010" target="_blank">Protocol S1</a>. Arrow indicates an increase in DNA content. (<b>B</b>) Quantification of cells shown in (<b>A</b>) in G0/G1, S and G2/M phases by flow cytometry and analysis with the CellQuest software, with peak of G0/G1 arbitrarily positioned upon acquisition at 200 on the linear scale of FL2-A X-axis for each sample (30000 total events acquired). (<b>C</b>) Representative images of Giemsa-stained metaphase chromosomes from mock-treated or 48 h-infected J774 (n = 75 for infected and 23 for mock-treated cells). (<b>D</b>) Representative images of Giemsa-stained metaphase chromosomes from mock-treated or 48 h-infected BMDM (n = 107 for infected and 18 for mock-treated cells). BMDM were cultured in presence of 30% CSF-conditioned medium from L929 cells and used directly without synchronization by M-CSF starvation. Values indicated are % of metaphases with the following number of chromosomes: Very low (8–27), low (29–36) and high (50–114). Scale bar 50 µm.