Effect of GILZ on TNF-α regulation of Osx and Runx2 expression.

<p>Real-time qRT-PCR and Western blot analyses of mRNA and protein expression. MSC-GFP and MSC-GILZ cells were cultured in OS with or without 1 ng/ml TNF-α (OST) for 8 days. At the end of treatment, one set of cells was harvested for real-time qRT-PCR (A, B) and a second set for Western blot (C) analyses of Osx and Runx2. Equal loading of samples in each lane is shown by the levels of β-actin. These experiments were performed at least three times with similar results. Quantitative results for levels of Osx and Runx2 protein are presented as bar graphs (D, E). Value of intensity for MSC-GFP cells cultured in regular DMEM growth medium (GM) is arbitrarily set as 1. Error bars indicate S.D. a, <i>p</i><0.01 (n = 3).