Apical cell surface abundance of MUC16 is reduced following treatment of epithelia with SP168 growth culture filtrate.

<p>Apical membrane levels of MUC16 were determined by cell surface biotinylation. <b>A</b>) Western blot analyses (using M11 antibody) of the amount of released and residual biotin-labeled surface MUC16 after exposure to medium only control or SP168 growth culture filtrate. <b>B</b>) Quantitative analyses of the western blot in (A) showing a reduction in the abundance of surface MUC16 following treatment with the SP168 culture filtrate (p<0.0001, Student's t-test, n = 3). Data for MUC16 surface abundance is expressed as a percentage of the total released plus surface MUC16 ± SEM.