Induction of the ARE-binding protein TTP in mitogen-stimulated cancer cell lines.

<p>(A) Schematic of <i>PIM1</i> mRNA showing a putative ARE sequence within the distal 3′UTR. Sequences corresponding to consensus TTP-binding sites are underlined. (B) Western blots showing the induction of TTP protein at selected times following mitogenic stimulation of cancer cell lines. Whole cell lysates were prepared at indicated time points following addition of serum+TPA to serum-starved cultures. GAPDH levels were used to normalize protein loading. The positions of molecular weight markers (in kDa) are indicated left of each blot.