Validation of potential tissue-specific and cancer antibody biomarkers by in situ hybridization on tissue arrays.

<p>ISH signals are seen as blue/purple staining with nuclei counterstained in methyl green, whereas IHC signals are in brown with hematoxylin counterstain. <i>A–C</i>: Bromodomain containing 1 (<i>BRD1</i>) in adrenal gland, <i>D–F</i>: Histone-lysine N-methyltransferase (<i>EZH2</i>) in appendix (nuclear and cytoplasmic staining for protein and mRNA, respectively), <i>G–I</i>: Junction plakoglobin (<i>JUP</i>) in skin, <i>J–L</i>: Special AT-rich sequence-binding protein 2 (<i>SATB2</i>) in colon (nuclear and cytoplasmic staining for protein and mRNA, respectively). Tissue arrays were hybridized with sense control probes (A, D, G, J), antisense probes (B, E, H, K), or immunostained with antibodies (C, F, I, L) targeting the respective transcripts or protein products. All images were derived from slides scanned with a 40× objective.