PTB and <i>let-7</i> miRNA contribute together to regulate gene expression in <i>C. elegans</i>.

<p>(A) Synchronized L1 animals were placed at semi-permissive temperature (20°C) and adult animals were scored after seventieth-two hours. The animal sterility observed in the population is caused by either a vulval bursting at the L4-adult transition or by a severe gonadal defect. Error bars represent the 95% confidence interval from independent experiments (n) where between 20 and 40 animals have been scored. ***: p<0.0001 (B) <i>let-7</i> level remained unchanged in the <i>let-7ts</i>/<i>ptb-1</i> animals. RNAs were purified from the indicated genotypes and probed for <i>let-7</i> and U6 RNAs. The amount of RNA was used for Northern blotting is indicated on the top of the panel and the U6/<i>let-7</i> ratios are presented at the bottom of the panel.