The markers of pre-migratory neural crest cells are down-regulated in <i>arl6ip1</i>-MO1-injected embryos.

<p>Dorsal views of 3-somite-stage wild-type (A, B, C, D) and <i>arl6ip1</i> knockdown embryos (E, F, G, H), or 8-somite-stage wild-type (I, J, K,) and <i>arl6ip1</i> knockdown embryos (L, M, N) were shown to reveal the indicated markers. Neural plate border was indicated with the expression of <i>tfap2α</i> (A, E), <i>foxd3</i> (B, F), <i>snai1b</i> (C, G) and <i>sox10</i> (D, H). Decreased expression of these markers, except <i>tfap2α</i>, was evident and predominant in <i>arl6ip1</i> morphants. Arrows indicate position of midbrain-hindbrain boundary. (I–K) At the 8-somite stage, these markers were expressed predominantly in early migrating cranial neural crest cells (indicated by brackets). (L–N) These genes were reduced in embryos co-injected with 4 ng <i>arl6ip1</i>-MO1/6 ng <i>p53</i>-MO, especially in <i>foxd3</i> and <i>snai1b</i> expression.