Enteric nervous system of <i>arl6ip1</i> morphants is degenerated.

<p>Wild-type (WT) embryos (A, D, G), <i>arl6ip1</i>-MO1-injected embryos (B, E, H), and <i>arl6ip1</i>-MO1/<i>p53</i>-MO-injected embryos (C, F, I) were observed under dorsal view at 36-hpf (A–C) and under lateral view at 48 hpf (D, E, F) and 72 hpf (G, H, I). Neural crest cells (NCCs), which were labeled by <i>crestin</i> probe (arrowheads in A), of WT embryos exited the vagal region and migrated to the enteric region. However, the <i>crestin</i>-labeled NCCs of <i>arl6ip1</i>-MO1-injected and <i>arl6ip1</i>-MO1/<i>p53</i>-MO-injected embryos remained in the vagal region (B, C). Compared to WT embryos, either the <i>arl6ip1</i>-MO1-injected embryos or <i>arl6ip1</i>-MO1/<i>p53</i>-MO-injected embryos exhibited shortened enteric neurons and delayed migration (D vs. E; D vs. F; distance between two arrows). Additionally, the <i>phox2b</i>-positive enteric neurons were distributed throughout the entire gut to the anus (G). Arrows indicate the most posterior region of migration, and the asterisks represent the anus. The enteric neurons of <i>arl6ip1</i>-MO1-injected embryos and <i>arl6ip1</i>-MO1/<i>p53</i>-MO-injected embryos all failed to reach as far as the anus (H, I).