The plasma membrane proteome of bone marrow-derived dendritic cells (DCs).

<p><b><i>Panel A:</i></b> Bone marrow-derived dendritic cells (BmDCs) were obtained by culturing bone marrow cells with GM-CSF. <b><i>Panel B:</i></b> Flow cytometric analysis of CD11c and F4/80 expression in BmDCs and BmMs. Results are presented as a contour plot with 10% probability increments. <b><i>Panel C:</i></b> Cell-surface exp ression of MHC-II by BmDCs and BmMs as assessed by flow cytometry. <b><i>Panel D:</i></b> The plasma membrane proteome of DCs. <i>Upper Panel:</i> Proteins expressed at similar levels by DCs and either M1 cells, M2 cells, or BmMs. <i>Lower Panel:</i> Proteins differentially expressed by DCs relative to M1 cells, M2 cells, and BmMs (<i>G-</i>test>1.5 or <−1.5 and <i>t-</i>test: <i>p</i><0.05). Red, upregulated; green, downregulated. <b><i>Panel E:</i></b> Examples of proteins expressed at similar levels by DCs and either M1 cells, or M2 cells. Results (N = 6 per group) are means and SDs. <b><i>Panel F:</i></b> Examples of plasma membrane proteins differentially expressed by DCs. Flow cytometry experiments are representative of 3 independent analyses.