Production of IgG by immunization with a complex of hTM4SF5R2-3 peptide and Lipoplex(O).

<p><b>A.</b> Optimal dose of peptide in the complex of hTM4SF5R2-3 peptide and Lipoplex(O). BALB/c mice (n = 3/group) were immunized with increasing amounts of hTM4SF5R2-3 peptide and Lipoplex(O) complex. <b>B.</b> Effect of the injection protocol. We immunized one group of mice with hTM4SF5R2-3 peptide after injection with Lipoplex(O) (Lipoplex(O)/TM4SF5R2-3) and another group with MB-ODN 4531(O) after injection with hTM4SF5R2-3 peptide and the DOPE∶CHEMS complex (DOPE∶CHEMS+TM4SF5/4531(O)) three times with a 10 day interval (n = 3/group). The sera were collected, and titers of the peptide-specific total IgG were assayed with an ELISA kit. These experiments were performed 3 times with similar results. **<i>P</i><0.01 (<i>vs</i> PBS control).