Secondary cell precursors are detectable in <i>dve</i> mutants.

<p>(A and B) Accessory glands of a control male (A: <i>dve^E181/CyOGFP</i>) and a <i>dve-A</i> mutant male (B: <i>dve^E181/dve^E144</i>) at 72 hr APF. Nuclei are labeled in magenta with anti-Prd antibody. The control secondary cell precursors (outlined in A′) strongly express Abd-B (green). A <i>dve-A</i> mutant accessory gland has a few secondary cell precursors in which Prd expression is greatly reduced (outlined in B′). (C and D) Accessory glands of 4-days old adults are labeled with anti-Prd (red) and anti-Abd-B (green) antibodies. (C) Loss of secondary cells in <i>dve-A</i> mutants is rescued by expressing <i>dve-A</i> (<i>w; dve^E181/dve^E144; dG30A/UAS-dveA-9B2</i>). (D) Loss of secondary cells in <i>dve-A</i> mutants is also rescued by expressing <i>dve-B</i> (<i>w; dve^E181/dve^E144; dG30A/UAS-dveB-Y4N</i>). The rescued secondary cells are outlined. They express Abd-B (white in C′ and D′) and have vacuole components that are non-specifically stained with anti-Prd antibody (red in C′ and D′).