Generation of <i>iafgp</i>-transgenic <i>D. melanogaster</i>.

<p>(A) Schematic representation of constructs used to generate <i>iafgp</i>-or <i>mcherry</i>-transgenic <i>D. melanogaster</i> is shown. (B) RT-PCR results showing expression of <i>iafgp</i> or <i>mcherry</i> transcripts in <i>iafgp</i>-or <i>mcherry</i>-expressing male (M) and female (F) flies. Reactions performed with no reverse transcriptase enzyme are shown as −RT. Levels of 28S gene transcripts were used as controls. Quantitative PCR results showing levels of <i>iafgp</i> (C) or <i>mcherry</i> (D) transcripts normalized to the levels of 28S gene transcripts in <i>iafgp</i>-or <i>mcherry</i>-expressing male and female flies.